If the assays give different but complementary results, evaluate plants at higher temperatures using both assays. dieffenbachiae using a transgenically-modified strain of Xad which contains the lux gene for bioluminescence b) Compare the above assay with an assay that uses a transgenically-modified strain containing the gene for green fluorescent protein (gfp c)Use the most efficient assay to evaluate plants under higher temperatures and higher inoculum levels to assess level of resistance. Project Methods The procedures will be to a)Develop and optimize a rapid and efficient protocol for screening anthurium plants for resistance to Xanthomonas axonopodis pv. Measurable outcomes of this project will be the production of new blight resistant anthurium cultivars which later will become available to the anthurium industry. This project will be integrated with a separate project 868H for delivery of plants to growers. dieffenbachiae.2)Optimize growth of stage IV microplants using nutritional and beneficial bacteria when microplants transition from axenic culture to community pots.3)Select resistant plants for further multiplication and advanced testing.OUTPUTS will be publications new methodologies for rapid and efficient evaluation of resistance in anthurium cultivars new blight resistant anthurium cultivars that can be available to local growers. Specific objectives:1)Develop and optimize a rapid protocol for screening anthurium plants for resistance to bacterial blight caused by Xanthomonas axonopodis pv. We aim to evaluate new hybrids developed by traditional breeding but the technology will be available for evaluation of transgenic plants as well. ![]() Goals / Objectives The overall goals of this project are to produce new anthurium cultivars resistant to bacterial blight and to accelerate the evaluations of disease resistance using the most efficient and up-dated technology. ![]() Reduced levels of bacterial blight disease on Hawaiian farms will impact the local economy. ![]() Application of the rapid screening procedures by the anthurium breeder is also an indicator of acceptance of the protocol. Outcome indicators will be grower evaluations of the plants generated in this program and put under natural disease pressure on Hawaiian farms. A successful project will have a positive impact both on anthurium growers and plant breeders who need faster methods of selecting anthurium-blight resistant plants. The projected outcome of the project will be increased availability of resistant anthurium plants in a more timely manner. We aim to accelerate the selection process using new molecular methods to evaluate and select the best candidates. They need an efficient and rapid assay for evaluating candidate plants. Researchers are working to accelerate time-consuming steps in the breeding process which involves selection of bacterial blight resistant Anthurium andreanum-type plants. Both traditional breeding and development of transgenic resistant plants requires efficient screening for selection and propagation of the most suitable cultivars. However, for long term growth of the industry and international competitiveness, growers rely on disease resistance. ![]() An integrated disease management program that includes production of clean planting stocks through tissue culture, sanitation, aeration and other cultural and biological control methods has reduced disease incidence to less than 5% on well-managed anthurium farms. Non Technical Summary Bacterial blight of anthurium is a persistant disease that causes plant death and production losses world wide.
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